一氧化氮比色分析試劑盒提供了一個只需要兩部，便可便捷的檢測檢測亞硝酸鹽離子和硝酸鹽離子濃度的方案。步，硝酸鹽離子被硝酸鹽還原酶轉(zhuǎn)化成亞硝酸鹽離子，然后，使用Griess試劑將亞硝酸鹽轉(zhuǎn)化成深紫色的含氮化合物。該含氮化合物內(nèi)發(fā)色團(tuán)的量反應(yīng)了檢測樣本內(nèi)NO的總量。該試劑盒檢測靈敏度為0·1nmole 亞硝酸鹽/孔或1μM亞硝酸鹽。該一氧化氮熒光分析試劑盒可用于哺乳動物細(xì)胞組織裂解液、細(xì)胞培養(yǎng)上清、血漿、血清、尿液以及組織勻漿內(nèi)一氧化氮含量的檢測。

Kit Summary:
• Detection method- Absorbance （540 nm）
• Sample type- Cell ａnd Tissue lysates, culture media, urine, plasma ａnd serum, as well as many other biological fluids
• Species reactivity- Mammalian
• Applications- BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process.

Features & Benefits:
• Simple two-step procedure; takes only ~1-1.5 hours
• Fast ａnd convenient
• BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite.

Kit components:
• Assay Buffer
• Enzyme cofactor
• Enhancer
• Nitrate Reductase
• Nitrate Standard
• Nitrite Standard
• Griess Reagent R1
• Griess Reagent R2
• Microtiter Plate
• Plate Cover

Description:
Nitric oxide （NO） plays an important role in neurotransmission, vascular regulation, immune response ａnd apoptosis. NO is rapidly oxidized to nitrite ａnd nitrate which are used to quantitate NO production. BioVision’s Nitric Oxide Colorimetric Assay Kit provides an accurate, convenient measure of total nitrate/nitrite in a simple two-step process. The first step converts nitrate to nitrite utilizing nitrate reductase. The second step uses Griess Reagents to convert nitrite to a deep purple azo compound. The amount of the azochromophore accurately reflects nitric oxide amount in samples. The detection limit of the assay is approximately 0.1 nmole nitrite/well, or 1 µM.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.