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【文獻(xiàn)標(biāo)題】Manipulation of hydrophobic motifs ａnd optimization of sequence patterns to design high stability peptides against piglet bacterial infections
【作者單位】中國農(nóng)業(yè)大學(xué)（China Agricultural University）
【文獻(xiàn)中引用產(chǎn)品】
豬白細(xì)胞介素6（IL-6）ELISA試劑盒
豬白細(xì)胞介素1β?(IL-1β)ELISA試劑盒
豬腫瘤壞死因子α（TNF-α）ELISA試劑盒
【關(guān)鍵詞】Antibacterial biomaterials ,High stability ,Manipulation of hydrophobic motifs ,Optimization of sequence patterns ,In vivo efficacy
【DOI】https://doi.org/10.1016/j.nantod.2023.101793
【影響因子(IF)】18.962
【出版期刊】《Nano Today》
【產(chǎn)品原文引用】
Healthy female weaned piglets (Duroc × Landrace × Large white, weighing ≈ 8 kg) were randomly divided into four groups (5 per group). One group of healthy piglets received no treatment ａnd served as a control. Piglets in the other three groups were intraperitoneally injected with 15 mL of E. coli ATCC25922 with an OD600 = 0.15, ａnd 2 h later, they were given an intraperitoneal injection of saline, peptide HLFP-5 (2.5 mg/kg), or HLWP-4 (2.5 mg/kg), respectively, with an injection volume of 10 mL. After 12 h, all piglets were euthanized. The blood of all piglets was collected ａnd the levels of inflammatory factors in serum were determined by the ELISA method. The inflammatory factor kit was purchased from Shanghai Hengyuan Biological Technology Co., Ltd. Partial livers, kidneys, spleens, ａnd lungs of bacteria-infected piglets were collected for bacterial enumeration studies by standardized localization. Furthermore, some organ samples of piglets were stained with H&E for histological examination.

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