先恭賀東北大學(xué)武老師使用上海恒遠(yuǎn)生物自主品牌ELISA試劑盒在《ACS Nano》（美國(guó)化學(xué)學(xué)會(huì)）期刊上發(fā)表的SCI文獻(xiàn)——Ratiometric 3D DNA Machine Combined with Machine Learning Algorithm for Ultrasensitive ａnd High-Precision Screening of Early Urinary Diseases順利通過(guò)，IF影響因子高達(dá)15.88分。同時(shí)恭賀武老師喜提上海恒遠(yuǎn)生物贈(zèng)送的獎(jiǎng)學(xué)金，正所謂雙喜臨門呀！

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【文獻(xiàn)標(biāo)題】Ratiometric 3D DNA Machine Combined with Machine Learning Algorithm for Ultrasensitive ａnd High-Precision Screening of Early Urinary Diseases
【作者單位】東北大學(xué)（Northeastern University）
【文獻(xiàn)中引用產(chǎn)品】
人CD63分子（CD63）ELISA試劑盒
【關(guān)鍵詞】uEVs ,DNA machine, fluorescence detection ,machine learning, urinary diseases
【DOI】https://doi.org/10.1021/acsnano.1c06429
【影響因子(IF)】15.88
【出版期刊】《ACS Nano》
【產(chǎn)品原文引用】
Materials.
All the oligonucleotides were synthesized ａnd purified by Shanghai Sangon Biotechnology Co. Ltd. (Shanghai, China), ａnd their sequences are listed in Table S1. All oligonucleotide stock solutions were prepared by dissolving DNA strands in Tris-HCl buffer (10 mmol L-1 Tris, 10 mmol L-1 MgCl2, pH 8.0) ａnd stored at -20 °C refrigerator before use. All the DNA strands were annealed at 95 °C for 5 min ａnd slowly cooled down to room temperature to obtain stable structures. 1-Ethyl-3-[3-(dimethylamino)-propyl] carbodiimide hydrochloride (EDC) ａnd N?hydroxysuccinimide (NHS) were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Sodium hydroxide (NaOH), Na2HPO4·12H2O, NaH2PO4·2H2O, AgNO3 ａnd KCl were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). 2-(N Morpholino) ethanesulfonic acid (MES), Gel-Red, loading buffer ａnd 5×TBE buffer were purchased from Sangon Biotechnology Co. Ltd. (Shanghai, China). BCA Protein Quantification Kit was provided by Beyotime Biotechnology (Shanghai, China). Dulbecco’s modified eagle medium (DMEM) was acquired from Aladdin Reagent Co. Ltd. (Shanghai, China). Carboxyl magnetic nanoparticles (COOH-MNPs, 200 nm) was purchased from PuriMag Biotech Co. Ltd. (Xiamen, China). Exo III, phi29 DNA polymerase were purchased from Sangon Biotechnology Co. Ltd. (Shanghai, China). T4 DNA ligase ａnd deoxynucleotides (dNTPs) were purchased from Takara Biotechnology Co. (Dalian, China). Thioflavine T (ThT) was obtained from Yuanye Bio-Technology Co. Ltd. (Shanghai, China). Anti?CD63 rabbit monoclonal antibody(ab193349) was purchased from Abcam Co. Ltd. (Shang, China). Anti-CD9 rabbit monoclonal antibody (20597-1-ap), anti-TSG101 rabbit monoclonal antibody (28283-1-AP) ａnd β-actin monoclonal antibody (66009-1-Ig) were purchased from Proteintech Group Co. Ltd. (Chicago, USA). ELISA (human CD63) kit was purchased from Shanghai Hengyuan Biological Technology (Shanghai, China). All chemicals were analytical grade ａnd directly used without further purification.

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