ELISA試劑盒 ELISA KIT  Elisa檢測試劑盒

產(chǎn)品名稱：人正常T細(xì)胞表達(dá)和分泌因子（RANTES/CCL5 ）

英文名稱：Human regulated on activation in normal T-cell expressed ａnd secreted,RANTES ELISA KIT

規(guī)格：96人份/48人份

包裝：進(jìn)口、國產(chǎn)、分裝、原裝

人正常T細(xì)胞表達(dá)和分泌因子（RANTES/CCL5 ）主要作用：科研

試劑盒種屬：人、大小鼠、豚鼠、豬、雞、兔、羊、猴、兔、馬...

待檢樣本：體液，血清，血漿，細(xì)胞培養(yǎng)上清液，尿液，組織勻漿,心房水標(biāo)本等等。

人正常T細(xì)胞表達(dá)和分泌因子（RANTES/CCL5 ）注意事項(xiàng)（中文）                                                    

1．試劑盒從冷藏環(huán)境中取出應(yīng)在室溫平衡15-30分鐘后方可使用，酶標(biāo)包被板開封后如未用完，板條應(yīng)裝入密封袋中保存。

2．濃洗滌液可能會有結(jié)晶析出，稀釋時可在水浴中加溫助溶，洗滌時不影響結(jié)果。

3．各步加樣均應(yīng)使用加樣器，并經(jīng)常校對其性，以避免試驗(yàn)誤差。一次加樣時間控制在5分鐘內(nèi)，如標(biāo)本數(shù)量多，推薦使用排槍加樣。

4．請每次測定的同時做標(biāo)準(zhǔn)曲線，做復(fù)孔。如標(biāo)本中待測物質(zhì)含量過高（樣本OD值大于標(biāo)準(zhǔn)品孔孔的OD值），請先用樣品稀釋液稀釋一定倍數(shù)（n倍）后再測定，計(jì)算時請乘以總稀釋倍數(shù)（×n×5）。

5．封板膜只限一次性使用，以避免交叉污染。

6．底物請避光保存。

7．嚴(yán)格按照說明書的操作進(jìn)行，試驗(yàn)結(jié)果判定必須以酶標(biāo)儀讀數(shù)為準(zhǔn).

8．所有樣品，洗滌液和各種廢棄物都應(yīng)按傳染物處理。

9．本試劑不同批號組分不得混用。

10.如與英文說明書有異，以英文說明書為準(zhǔn)。                              

Important notes（英文）                                                             

1.The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

2.washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

3.add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .

4.if the testing material content is excessively higher （The sample OD is bigger than the first standard well ）,please dilute Sample （n-fold）, Please diluente ａnd multiplied by the dilution factor.（×n×5）.

5.Closure plate membrane only limits the disposable use, to avoid cross-contamination.

6.The substrate evade the light preservation.

7.Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

8.All samples, washing buffer ａnd each kind of reject should according to infective material process.

9.Do not mix reagents with those from other lots.

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